a kinase anchoring protein 4 Search Results


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ELISA for estimation of Human A-Kinase anchor Protein 4, AKAP4 in serum, plasma and other biological fluids
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Proteintech akap4
A.Examination of selective IFT protein expression levels in the control and conditional Ift74 knockout mice by Western blot. Compared to the controls, the expression levels of IFT27, IFT57, IFT81, IFT88 and IFT140, but not IFT20 and IFT25 are reduced in the conditional Ift74 knockout mice. a: representative Western blot result; b: quantitative analysis of selective IFT protein expression. B. Examination of testicular expression levels of ODF2, a component of sperm tail outer dense fibers, <t>AKAP4,</t> a major component of fibrous sheath, and SPAG16L, a component of axonemal central apparatus protein in the control and conditional Ift74 knockout mice. There was no difference in ODF2 and SPAG16L expression. However, the processed AKAP4 was significantly reduced in the conditional Ift74 knockout mice. β-ACTIN was used as controls. a: representative Western blot result; b: quantitative analysis of ODF2 and SPAG16L; c: quantitative analysis of AKAP4.
Akap4, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+kinase+anchoring+protein+4/AKAP4+Antibody/bio_rxiv__457804-143-83-98
Average 93 stars, based on 1 article reviews
akap4 - by Bioz Stars, 2026-10
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94
Shanghai Korain Biotech Co Ltd human a-kinase anchor protein 4
A.Examination of selective IFT protein expression levels in the control and conditional Ift74 knockout mice by Western blot. Compared to the controls, the expression levels of IFT27, IFT57, IFT81, IFT88 and IFT140, but not IFT20 and IFT25 are reduced in the conditional Ift74 knockout mice. a: representative Western blot result; b: quantitative analysis of selective IFT protein expression. B. Examination of testicular expression levels of ODF2, a component of sperm tail outer dense fibers, <t>AKAP4,</t> a major component of fibrous sheath, and SPAG16L, a component of axonemal central apparatus protein in the control and conditional Ift74 knockout mice. There was no difference in ODF2 and SPAG16L expression. However, the processed AKAP4 was significantly reduced in the conditional Ift74 knockout mice. β-ACTIN was used as controls. a: representative Western blot result; b: quantitative analysis of ODF2 and SPAG16L; c: quantitative analysis of AKAP4.
Human A Kinase Anchor Protein 4, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+kinase+anchoring+protein+4/Human+A-kinase+Anchor+Protein+4/custom%40e4917hu%4010%2E1002%2Fpnp%2E835
Average 94 stars, based on 1 article reviews
human a-kinase anchor protein 4 - by Bioz Stars, 2026-10
94/100 stars
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90
Florman Family Foundation a-kinase anchoring protein 4
A.Examination of selective IFT protein expression levels in the control and conditional Ift74 knockout mice by Western blot. Compared to the controls, the expression levels of IFT27, IFT57, IFT81, IFT88 and IFT140, but not IFT20 and IFT25 are reduced in the conditional Ift74 knockout mice. a: representative Western blot result; b: quantitative analysis of selective IFT protein expression. B. Examination of testicular expression levels of ODF2, a component of sperm tail outer dense fibers, <t>AKAP4,</t> a major component of fibrous sheath, and SPAG16L, a component of axonemal central apparatus protein in the control and conditional Ift74 knockout mice. There was no difference in ODF2 and SPAG16L expression. However, the processed AKAP4 was significantly reduced in the conditional Ift74 knockout mice. β-ACTIN was used as controls. a: representative Western blot result; b: quantitative analysis of ODF2 and SPAG16L; c: quantitative analysis of AKAP4.
A Kinase Anchoring Protein 4, supplied by Florman Family Foundation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+kinase+anchoring+protein+4/a+kinase+anchoring+protein+4/pm25735235-128-19-49
Average 90 stars, based on 1 article reviews
a-kinase anchoring protein 4 - by Bioz Stars, 2026-10
90/100 stars
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Human A-kinase anchor protein 4(AKAP4) ELISA kit
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Boster Bio Anti-A-kinase anchor protein 4 AKAP4 Antibody (Catalog # A07362). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
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A.Examination of selective IFT protein expression levels in the control and conditional Ift74 knockout mice by Western blot. Compared to the controls, the expression levels of IFT27, IFT57, IFT81, IFT88 and IFT140, but not IFT20 and IFT25 are reduced in the conditional Ift74 knockout mice. a: representative Western blot result; b: quantitative analysis of selective IFT protein expression. B. Examination of testicular expression levels of ODF2, a component of sperm tail outer dense fibers, AKAP4, a major component of fibrous sheath, and SPAG16L, a component of axonemal central apparatus protein in the control and conditional Ift74 knockout mice. There was no difference in ODF2 and SPAG16L expression. However, the processed AKAP4 was significantly reduced in the conditional Ift74 knockout mice. β-ACTIN was used as controls. a: representative Western blot result; b: quantitative analysis of ODF2 and SPAG16L; c: quantitative analysis of AKAP4.

Journal: bioRxiv

Article Title: Intraflagellar transport protein 74 is essential for mouse spermatogenesis and male fertility by regulating axonemal microtubule assembly in mice

doi: 10.1101/457804

Figure Lengend Snippet: A.Examination of selective IFT protein expression levels in the control and conditional Ift74 knockout mice by Western blot. Compared to the controls, the expression levels of IFT27, IFT57, IFT81, IFT88 and IFT140, but not IFT20 and IFT25 are reduced in the conditional Ift74 knockout mice. a: representative Western blot result; b: quantitative analysis of selective IFT protein expression. B. Examination of testicular expression levels of ODF2, a component of sperm tail outer dense fibers, AKAP4, a major component of fibrous sheath, and SPAG16L, a component of axonemal central apparatus protein in the control and conditional Ift74 knockout mice. There was no difference in ODF2 and SPAG16L expression. However, the processed AKAP4 was significantly reduced in the conditional Ift74 knockout mice. β-ACTIN was used as controls. a: representative Western blot result; b: quantitative analysis of ODF2 and SPAG16L; c: quantitative analysis of AKAP4.

Article Snippet: Then the Nonspecific sites were blocked in a Tris-buffered saline solution containing 5% nonfat dry milk powder and 0.05% Tween 20 (TBST) for 1 hour at room temperature, and the membranes were incubated with indicated primary antibodies (IFT25: 1:2000, Cat No: 15732-1-AP, ProteinTech; IFT74: 1:2000, Cat No: AAS27620e from ANTIBODY VERIFY; IFT81: 1:1000, Cat No: 11744-1-AP, ProteinTech; β-actin: 1:2000, Cat No: 4967 S, Cell Signaling Antibodies against IFT20, IFT27, IFT57, IFT88 and IFT140 (1:2000) were from Dr. Pazour’s laboratory ( , , ); AKAP4: 1:4000, from Dr. George Gerton at University of Pennsylvania; ODF2: 1:800, Cat No: 12058-1-AP, ProteinTech; SPAG16L: 1:1000, generated by Z.Z.’s laboratory) at 4°C overnight.

Techniques: Expressing, Knock-Out, Western Blot